Significant finding of Bordetella holmesii DNA in nasopharyngeal samples from French patients with suspected pertussis.
نویسندگان
چکیده
Pertussis is routinely diagnosed with real-time PCR based on insertion sequence IS481, which is not specific for Bordetella pertussis. We conducted a retrospective study using real-time PCRs specific for Bordetella pertussis and for Bordetella holmesii on 177 samples positive for IS481 PCR. Bordetella holmesii DNA was detected in 20.3% samples collected from adolescents and adults.
منابع مشابه
Bordetella holmesii in nasopharyngeal samples from Chilean patients with suspected Bordetella pertussis infection.
We read with interest the comment of C. Miranda, L. Porte, and P. Garcia contributing to the problem of real-time PCR biological diagnosis of whooping cough using IS481 as the target. The authors detected Bordetella holmesii in 7 patients out of 51 between 0 and 9 years of age, with 3 being less than 12 months old. It would have been of interest to know the ages of the 4 other patients. The maj...
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1. Njamkepo E, Bonacorsi S, Debruyne M, Gibaud SA, Guillot S, Guiso N. Significant finding of Bordetella holmesii DNA in nasopharyngeal samples from French patients with suspected pertussis. J Clin Microbiol. 2011;49:4347–8. http://dx.doi. org/10.1128/JCM.01272-11 2. Weyant RS, Hollis DG, Weaver RE, Amin MFM, Steigerwalt AG, O’Connor SP, et al. Bordetella holmesii sp. nov., a new gram-negative ...
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Bordetella holmesii, an emerging pathogen, can be misidentified as Bordetella pertussis by routine polymerase chain reaction (PCR). In some reports, up to 29% of the patients diagnosed with pertussis have in fact B. holmesii infection and invasive, non-respiratory B. holmesii infections have been reported worldwide. This misdiagnosis undermines the knowledge of pertussis' epidemiology, and may ...
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ورودعنوان ژورنال:
- Journal of clinical microbiology
دوره 49 12 شماره
صفحات -
تاریخ انتشار 2011